Abstract
We previously reported in this meeting that the rice gene responsible for cpm1 mutation, which results in a defect in phytochrome-mediated inhibition of coleoptile growth, encodes an allene oxide synthase (OsAOS1). Sequence analysis indicated that the cpm1 mutant expresses a full-length OsAOS1 in which one amino acid is substituted. Using recombinant proteins, we confirmed that the wild-type OsAOS1 has AOS activity, but the point-mutated OsAOS1 has little activity. We further showed that both wounding and red light induce an enhancement of jasmonate level in wild-type coleoptiles, but only a slight enhancement in cpm1 coleoptiles. A rice microarray was used to investigate wound- and red light-regulated genes. In wild type coleoptiles, expressions of more than 5000 and 6000 genes were regulated by wounding and red light, respectively. About 2000 genes were regulated by both treatments. The cpm1 mutant was used to show that many of these common genes are jasmonate-dependent.
