Abstract
Plants take many environmental changes around itself and managing to do by expressing some sets of genes. We focused the His-Asp phosphorelay signal transduction system of Oryza sativa and trying to study the functional characteristics of this system. This system was composed from 3 kinds of signaling factors, Histidine Kinase: HK, Response Regulator: RR, Histidine-containing phosphotransfer: HPt, by looking over the Rice genome information as reported in other higher plants, Arabidopsis and Zea mays. And each has some members. Here by using the yeast two-hybrid system, we studied the interaction between 2 OsRRs and 2 OsHPt signaling factors and furthermore which domain of each factor functioned on signaling.