Abstract
The moss Physcomitrella patens development starts from a germinating spore that gives rise to a filamentous structure called protonema. Bud that is arisen form protonema develops to give rise to a leafy shoot called gametohpore. After dissection of a leaf from a gametophore, leaf cells facing to the dissected cells change to protonemal cells. However, the molecular mechanisms of this process have been unknown. To understand the mechanisms, we focused on the cell cycle activation in leaf cells after leaf dissection. Our analysis with RT-PCR demonstrated that transcripts encoding several cell-cycle regulators, such as CYCLINs, increased in dissected leaves. Furthermore, we found that the CDK activity in dissected leaves increased after leaf dissection. Taken together, our results indicate that the CYCLIN expression and the CDK activation by leaf dissection are associated with cell cycle progression in leaf cells facing to the dissected leaf cells.
