Abstract
For revealing the mechanisms underlying the differentiation of chloroplast, reporter genes were placed under the control of the promoter of photosynthesis gene RBCS-3B, and subjected to the activation tagging to find callus expression of RBCS (CES) genes which promoted the expression of photosynthesis genes in the dedifferentiated calli (Plant Cell Physiol., 47, 319-331, 2006). Contrary to the functions of CES genes, it has been tried to hunt genes for depression of the expression of photosynthesis genes in greened calli.
We have established the culture condition under which calli become green. Mutants called des (depressed expression of RBCS) in which green calli change into white by activation tagging, have been selected. DNA prepared from des mutants was subjected to thermal asymmetric interlaced (TAIL)-PCR, resulting in identification of 4 loci. Gene expression in des mutants has been analyzed by real-time RT-PCR and Arabidopsis ATH1 Genome Array (Affymetrix).
