Abstract
In arabidopsis seeds, storage proteins are accumulated in the storage vacuoles from rough ER through vesicle trafficking pathway. In the mutant seeds deficient in the vacuolar sorting machinery, for example atvsr1 and atvps29, seed storage proteins are secreted and accumulated in outer cellular spaces.
Recently we developed an efficient and simple method for isolating vacuolar sorting mutants of Arabidopsis. In this method, we could easily isolate the mutant seed using GFP fluorescence as an indicator. Using this method, we obtained a lot of vacuolar sorting mutants, which were named gfs ( green fluorescent seed) mutants.
We mapped some of the novel gfs mutants, gfs4, gfs5 and gfs6, to different loci, including AtVSR1 and atvps29 genes. From further analysis, it is suggested that they may form the heteromeric protein complex.
We will report to identify novel machinery for vacuolar sorting of proteins.
