Abstract
We isolated a dominant mutant, cryptic precocious-1D (crp-1D) which dramatically enhances the precocious-flowering phenotype of 35S::FT. To gain insights into its function, we analyzed morphological phenotype of crp-1D mutant and loss-of-function mutants (crp-3, crp-4) which were obtained from the Salk T-DNA insertion collection. crp-1D mutant showed slightly early-flowering phenotype compared with wild type. By contrast, crp loss-of-function mutants showed late-flowering phenotype. Interestingly, crp mutants also showed pleiotropic phenotype. Seedlings of crp-1D were larger than wild type, whereas those of loss-of-function mutants were smaller than wild type. CRP encodes an Arabidopsis counterpart of Med12, a subunit of the transcriptional mediator complex. CRP::GUS was strongly expressed in vascular tissue and apical meristems. We are currently comparing gene expression profiles between crp mutants and wild type. Results of these analysis will be presented.
