Abstract
There is high proportion of transcription factors (TFs) in Arabidopsis genome compared to other organisms so that TFs play pivotal role for regulation of various biological processes in Arabidopsis. To identify the functions of transcriptional factors, we have tried to clone all annotated transcriptional factors without stop codon. Until now approx. 1000 transcriptional factors were cloned into entry vector by Gateway technology. This collection is thought as good resource because the clone is easily move to objective destination vectors by LR reaction.
Using this collection, we are establishing novel Arabidopsis transgenic plants over-expressing independent transcription factors which is fused to glucocorticoide receptor for functional analysis. In this system, by application of a steroid hormone, dexamethasone (DEX), introduced transcriptional factor is functionally active. We have performed phenotypical observation of this mutant population after DEX application.
Here we show the detail of these lines and discuss the usefulness for functional genomics.
