Alterations of hitsone modifications on the stress-inducible genes under drought stress conditions in Arabidopsis

Abstract

Modulation of histone N-tail modifications correlates with transcriptional activation and repression in eukaryote. In plant, histone modifications are altered in vernalization process. However, no clear correlations between the status of histone modifications and the stress-inducible genes under abiotic stresses have been reported.
We have studied alterations of the histone modification patterns under abiotic stress conditions in Arabidopsis. We detected temporal changing of enrichment at methylation and acetylation sites in histone N-tail on the drought stress responsive genes, DREB2A, RD20, RD29A, RD29B and RAP2.4, under drought stress condition by Chromatin Immuno Precipitation assay. On the coding regions of these genes, K4me3 enrichments increased with drought stress treatment. We will discuss about the correlation between gene activation and histone status of histone H3 under drought stress conditions.