Abstract
Effects of light and glucose on the expression of soluble proteins were analyzed by means of two-dimentional electrophoresis in Synechocystis sp. PCC6803 which exibits not only photoautotrophic growth but also light-activated heterotrophic growth (LAHG) under dark with glucose as a carbon source. Various proteins were induced by light in the presence of glucose, while glucose addition in the dark was not effective for induction of many proteins, such as Fructose-1,6-bisphosphate aldolase (FBA). Therefore, both light and glucose were required for the full induction of protein synthesis.
One of the response regulators, sll1330, was demonstrated to be involved in regulation of FBA expression by light and glucose. FBA was synthesized only slightly under LAHG conditions in the disruptant of this gene. The disruptant of sll1330 grew scarcely in heterotrophic growth under intermittent light but did normally in autotrophic condition. These results suggest that sll1330 is the key regulator of FBA expression.
