Abstract
Acylamino Acid-Releasing Enzyme (AARE) is protease conserved widely among microorganism, mammal and plant. AARE catalyzes the N-terminal hydrolysis of Nα-acylpeptide, which releases Nα-acylated amino acids. It was found in vitro that AARE degraded oxidized and glycated protein. We analyzed plant AARE function in the degradation of oxidized protein using transgenic Arabidopsis thaliana.
Arabidopsis overexpressing AARE was generated using 35SCaMV-promoter and AARE silencing Arabidopsis was also created using RNAi. AARE expressions were confirmed by western blot and measuring AARE activity. Oxidative stress tolerance and levels of oxidized proteins were compared between wild-type (Col-0) and transgenic plant.
AARE silencing plants are more sensitive to oxidative stresses than wild-type one. The oxidized protein levels increased as AARE activity decreased.
