Abstract
The Β-type carbonic anhydrase (PtCA1) localizes as clustered particles at the surface of the girdle lamellae in the chloroplast of the marine diatom Phaeodactylum tricornutum. PtCA1 has been suggested to play important role in CCM but details of PtCA1 particle isn't known. PtCA1 cluster is formed only in the chloroplast, therefore this cluster is probably requires some aid of unknown stromal factors. In this study, we attempted to detect stromal factor. Rubisco was first thought to be the factor and thus localized using GFP laveling method. However, Rubisco localized at the stroma didn't form particles. LciB, a protein which is thought to be essential for CCM in the green algae localizes as cluster around the pyrenoid of C. reinhardtii. P. tricornutum has a homologue of LciB gene (PtLciB), which was cloned and fused with the egfp. Localization of PtLciB and results from immunoprecipitation assay by PtCA1 antibody will be discussed.
