Abstract
VOZ(Vascular plant One Zinc-finger protein) is a novel one-zinc finger type transcription factor family, which is highly conserved through vascular plants as well as a moss(Mitsuda et al.,45, 845-854, 2004). Arabidopsis harbors two VOZ homologs (AtVOZ1 and AtVOZ2) in the genome. The atvoz1/ atvoz2 double knockout plant shows the late-flowering phenotype under long day condition, suggesting that VOZ controls the photoperiodic flowering response. However, detail of the molecular mechanism (e.g. target genes) remains to be clarified. In this work, we performed a yeast two-hybrid screen by using AtVOZ1 or AtVOZ2 as baits, and obtained hundreds of candidates interacting with AtVOZ1 and/or AtVOZ2. in vitro and in planta binding assays revealed the existence of specific interacting factors, including transcriptional regulators which might co-operate with VOZ.
