Global Analysis for COI1- dependent and independent Gene Expression in Jasmonate Signaling Pathway

Abstract

Jasmonic acid (JA) is a phytohormone which has roles in regulating stress response and development. COI1 (CORONATINE INSENSITIVE 1) is an F-BOX protein working in ubiquitin-proteasome system. Most of JA responsive genes (JRGs) are regarded to be regulated by the COI1 complex. 12-Oxo-phytodienoic acid (OPDA) is a precursor of JA and also has certain role inducing COI1-independent gene expression in contrast to JA. Some OPDA responsive genes (ORGs) are confirmed to be induced without the COI1 complex.We analyzed gene expression responses to JA and OPDA under coi1 background using GeneChip and confirmed COI1-dependent and COI1-independent expression for JRG and ORGs. However, from 342 JRGs, two gene groups which show similarity in expression profiles between WT and coi1 mutant were obtained. More detailed analysis for COI1-independent JA responsive genes will be introduced in this presentation.