SD5, a homologue of spliosome subunit, is essential for post-seedling development

Abstract

In RIKEN PSC, 18,000 transposon insertion mutants of Arabidopsis were established. From these transposon tagging lines, several seedling lethal mutants were identified and we named segregation distortion (sd). One of them, sd5 did not develop mature leaves and this phenotype was caused by transposon inserting in At3g24730.
The protein encoded by At3g24730 is homologous to DIM2, a component of spliosome. Animal and plants have a DIM family comprised of DIM1 and DIM2, but yeast has only DIM1. Loss of DIM1 caused lethal as a result of defect of cell cycle in S.pombe. SD5 protein did not complement to S.pombe dim1-35 mutation when SD5 was expressed at limited temperature although Arabidopsis DIM1 complemented to this. This result show SD5 has defferent functions of DIM1 protein.
Here we show the detail of sd5 phenotypes and the SD5 functions.