Abstract
A mutation in CRL of Arabidopsis thaliana causes a severe defect in plastid division. Besides the inhibition of plastid division, the mutation causes distortion of cell division planes and abnormality in cell differentiation (Plant J., 38, 448-459, 2004). Our previous data indicated that CRL protein localized in the outer envelope membrane of plastids. In this study, we have reexamined the subcellular localization of the CRL protein using the transgenic lines expressing a CRL-GFP fusion protein that can complement crl mutation. Our results indicated that the CRL-GFP protein localized plastid periphery and the intensity of the signal was not uniform. The pattern of the CRL-GFP localization in dividing chloroplasts was the same as that of non-dividing ones. We are now investigating the subcellular localization of the CRL-GFP in roots and developing leaves under the normal condition and stress conditions. These results will be presented.
