Abstract
The SNF1-related protein kinase 2 (SnRK2) family are activated by osmotic stress and/or abscisic acid (ABA), and they contains 10 members in Arabidopsis genome (SRK2A–J). Recently, some SnRK2s were analyzed, for example, SRK2E/OST1 is activated by ABA and positively regulates ABA-dependent stomatal closure, or other two kinases, SRK2D and SRK2I, regulate ABA signaling in seeds and vegetative tissues. Since SnRK2s are activated by both ABA and/or osmotic stress, they are regarded as a hub module in stress signaling. However, the mechanism for SnRK2-mediated signal transduction is still unclear, therefore we have started a project to identify SnRK2-interacting proteins and analyze their roles in signaling networks. We have taken three strategies as follows: 1) Yeast two-hybrid screening, 2) Co-immunoprecipitation and 3) Phospho-proteomic analysis. Although this project on the systematic analysis of SnRK2 regulatory networks is still under way, some topics or progress will be introduced in this session.
