Abstract
We identified anthocyanins in the petals of tartary buckwheat to study physiological roles of them. As materials, we used a tartary buckwheat leading-variety and a mutant-line, which accumulates high amount of anthocyanins. Anthocyanins were identified using HPLC-ESI-MS/MS system. The leading-variety contained Cyanidin-3-Orutinoside (C3r) as a major anthocyanin, and also contained trace amount of Cyanidin-3-O-glucoside (C3g). The mutant-line contained C3r as a major anthocyanin, and also contained C3g, and trace amount of Cyanidin-3-O-galactosyl-rhamnoside. The total anthocyanin content in the mutant-line was 10 times higher than that of the leading-variety. On the other hand, we also measured Cyanidin content in methanol non-extractive proanthocyanidin (ProA). The leading-variety and the mutant lines contained 257 and 8.8 times higher amount of cyanidin moiety in ProA than in anthocyanins. From this finding, Cyanidin moieties may be distributed competitively between anthocyanins and ProA. We will study physiological roles of anthocyanins in tartary buckwheat petals using the mutant-line.
