Abstract
Resveratrol is a phytoalexin against Botrytis cinerea and one of the functional compounds for human health. Resveratrol is a member of terpenoids and has a stilbene chemical backbone. For terpenoid transport, PDR transporters, which belong to ABC transporters, had been reported in Nicotiana plumbaginifolia (NpABC1) and Spirodela polyrrhiza (SpTUR2). Thus we predicted the existence of PDR transporter for resveratrol secretion in grape berry skin. We found an orthologue (VvPDR1) of NpABC1 in grape genome (Genescope) and cDNA of VvPDR1 was cloned with total RNA from grape skin by RT-PCR. Closest homologue to VvPDR1 in Arabidopsis PDRs is AtPDR12, which transports sclareol. Resveratrol accumulation in grape berry skin is increased by ultraviolet (UV) irradiation, thus we treated UV to grape berry and VvPDR1 expression in the skin was determined. Gene expressions of both VvPDR1 and stilbene synthase was induced by UV, suggesting the involvement ofVvPDR1 in resveratrol accumulation in grape skin.
