Abstract
Blue light, perceived by blue light photoreceptor phototropins (phot1 and phot2), induces stomatal opening through the activation of plasma membrane H+-ATPase in guard cells. However, little is known about the signaling pathway from phototropins to the H+-ATPase. In this study, we isolated the mutants, which show closed stomata phenotype, from EMS-treated Arabidopsis by thermal imaging. First we established the condition for screening to distinguish leaf temperature from phot1 phot2 double mutant and wild type. Then, we performed the screening of mutants under above conditions. We isolated 10 mutants, which show closed stomata phenotype, from 40,000 plants. One of them was rpt2, which acts on the downstream of phot1 in phototropism and stomatal opening, suggesting that this screening is useful for isolation of stomatal aperture mutants in the signal transduction pathway of phototropin. In addition, we isolated open stomata mutants by this screening. We will also report these mutants.
