Abstract
Characterization of cross talk among the various stresses is quite important to know the multi response mechanisms for the environmental stresses. Recently, we have been studying the multi-response genes, AtGST1 and AtGST11, encoding Arabidopsis gluthathion S-transferase as model systems. In this study, we tried to isolate transcription factors (TF) which are related to the gene-expression of the two GST genes under aluminum (Al) stress by the two independent methods, Bio-panning and Yeast one hybrid system. By the former strategy, three candidates including Putative bZIP Transcription Factor and Homeobox-leucine zipper protein 6 were isolated. In case of the latter strategy, 8 of putative TF binding sites existing in the AtGST1 and 11 promoters were selected by computer analysis (TRANSFACT) and used for our TF screening. Thirteen of candidates including a putative Zinc finger protein and ethylene response element binding factor were obtained in this screening.
