Detection And Characterization Of Stress-Inducible Proteins In Oat Leaves

Abstract

In oats, it has been well known that defense responses against infection of pathogen and elicitor treatment involved various chemical changes such as synthesis of phytoalexins. However, little is known about the dynamic changes in protein content and composition during defense response. Here, we report accumulation of stress-inducible proteins in oat leaves after elicitor treatment and mechanical wounding. Two-dimensional gel electrophoresis showed that stress-inducible proteins after elicitor treatment accumulated in intercellular space and cytoplasm. We also demonstrated that these proteins were also produced in wounded leaves. These results suggest that defense against both elicitor and wounding share the same defense mechanisms in oats. Furthermore, to investigate physiological roles of stress-inducible proteins in disease resistance, we identified their selected proteins using LCMS-IT -TOF, showing that they were correspondent to those reported to be involved in defense mechanisms in many plant species.