Abstract
It has been established that the jasmonic acid (JA) is involved in the plant resistance to insect and pathogen attacks as well as anther dehiscence and senescence. However, little is known about the mechanism how the genes of JA biosynthetic pathway are induced by the attacks or wounding. We have found that the expression of Arabidopsis DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1) gene, which encodes a lipase responsible for the JA biosynthesis in stamen filaments at the anther dehiscence, is immediately induced in rosette leaves when the leaves are wounded. Recently, it was revealed that the DAD1 is also involved in the wound induction of JA. These findings strongly motivated us to determine the cis-regulatory element of DAD1 wound induction. The promoter-GUS analyses revealed that the element is located at the 3'-region of the gene.