Abstract
Ionizing radiation induces DNA damages which cause mutation. Organisms have DNA repair systems to repair DNA damages. The relationship between types of mutation induced by ionizing radiation and DNA repair systems are well studied in bacteria and animal. In plant, however, little is known about it. In this study we will investigate ion-beam and gamma-rays induced mutations in plant by using Arabidopsis mutation detection system (Yoshihara et al. 2006). Ion-beam and gamma-rays are irradiated to Arabidopsis containing E. coli rpsL gene. Mutated rpsL gene is collected as a plasmid by plasmid rescue from chromosomal DNA and analyze the types of mutations. We will report the results of ion-beam and gamma-rays induced mutations detected by this system.
