Abstract
A miniature cultivar Micro-Tom has attracted much attention as a model of tomato (Solanum lycopersicum). We have constructed five cDNA libraries and three full-length cDNA libraries from Micro-Tom, and generated ESTs and full-length cDNAs. By assembling Micro-Tom ESTs with publicly available tomato ESTs, a non-redundant consensus sequence set (unigenes) is obtained. A database MiBASE (http://www.kazusa.or.jp/jsol/microtom/) has been creating and maintaining to provide information of the Micro-Tom ESTs and tomato unigenes. The current version of MiBASE contains information of DNA markers, BLAST annotations, Gene Ontology terms, metabolic pathways, and gene expressions obtained from Micro-Tom cDNA microarrays. To elucidate intron-exon structures of the tomato genome, we also compare the full-length cDNAs with genomic sequences provided from the international tomato genome sequencing project. All information of the full-length cDNAs and genome annotations is available from a database KaFTom (http://www.pgb.kazusa.or.jp/kaftom/).
