Abstract
In Arabidopsis thaliana, many genes regulate leaf development through several pathways. The loss-of-function mutations in the ASYMMETRIC LEAVES2 (AS2) genes of Arabidopsis thaliana cause pleiotropic phenotypes in leaves, such as downward curling of leaves, reduced complexity of leaf venation pattern, generation of lobes and defect in the leaf adaxial-abaxial polarity. Our expression analysis suggests that AS2 represses directly or indirectly class 1 knox genes and abaxial factor genes, ETTIN, KANADI2, YABBY5. But the mechanisms for regulating the abaxial factor genes are unknown. To identify new components that function together with AS2 in leaf development, we performed a genetic screen for enhancers and suppressors of the as2-1 mutant. #27, one of enhancer mutants formed filament-like leaves that might be associated with defect in the adaxial-abaxial leaf polarity. We will present characteristic and genetic mapping of the #27 mutant.
