Abstract
The sliding theory of cytoplasmic streaming was proposed about 50 years ago. It is suggested that cytoplasmic streaming is caused by the motor protein, myosin, associated with streaming organelles actively sliding along actin filaments, with the hydrolysis energy of ATP. In GFP-h, which is a transgenic Arabidopsis thaliana expressing green fluorescent protein (GFP) with an endoplasmic reticulum (ER)-retention signal, the ER networks have been visualized together with ER bodies. We focused on the dynamic movements of the ER structures. The ER movement was inhibited by the treatment of an actin depolymerizing reagent or an inhibitor of myosin activity. To identify the Arabidopsis myosin(s) involved in the ER dynamics, we analyzed the ER movement in the myosin XI mutants. We will show and discuss about the involvement of myosin XI in the ER dynamics.
