Abstract
Photosystem I (PSI) complex consisting of 14-15 core subunits is conserved among higher plants and the green alga Chlamydomonas reinhardtii. In contrast, the number of Light-harvesting Complex I (LHCI) subunit is different; higher plants and Chlamydomonas contain 4-6 and 9 distinctive LHCI subunits, respectively. It remain elusive how 9 LHCI complexes are organized in Chlamydomonas. Here, antibodies against PSI and LHCI subunits were generated and used for analyzing LHCI structure by chemical cross-linking. We detected cross-linked products between PsaG-Lhca9, Lhca9-Lhca2, PsaK-Lhca3, PsaF-Lhca8, Lhca8-Lhca4, Lhca5-Lhca6. Based on the cross-linking experiments, we will discuss on oligomeric structure of LHCI in Chlamydomonas.
