Host: The Japanese Society of Plant Physiologists
Pages 0058
Nitrogen (N) is one of the most important macro elements for plant production, and urea is most frequently used N fertilizer worldwide. Recent studies suggested that urea can be directly taken up by plant in the form of urea.
We investigated AtDUR3, homologous gene for high-affinity urea transporter (DUR3) in budding yeast, in order to clarify its biological functions in Arabidopsis. The uptake of stable isotope labeled urea in T-DNA insertion lines for AtDUR3 was decreased compared with wild-type. Immunological analysis as well as promoter analysis showed the accumulation of AtDUR3 in the plasma membrane of roots under N deficient condition (Kojima et al., 2007). Recently Hem et al., 2007 reported identification of phosphorylation site in AtDUR3. We substituted potential phosphorylation site in AtDUR3 by site directed mutagenesis approach in order to mimic phosphorylation or dephosphorylation. Potential post-translational regulation of AtDUR3 will be discussed.
