Host: The Japanese Society of Plant Physiologists
Pages 0061
Peptides encoded by short open reading frame (sORF) are reported to be involved in various functions in yeast and Drosophila. We identified 7,159 sORFs ranging from 90 to 300 bp in the intergenic regions of the Arabidopsis genome. We developed an agilent custom microarray including 5,921 sORFs and 26,192 AGI genes (TAIR7) in Arabidopsis to identify sORF function. Expression of AGI genes and intergenic transcripts were detected in about 70% and 56% of total spot number, respectively. On the other hand, expression of about 9% sORF was detected. Some sORF expressions were confirmed by RT-PCR analysis to validate microarray results. RT-PCR analysis was preformed in various tissues (Leaf, Flower, Silique and Root). Some sORF showed tissue specific expression pattern. We will analyze gene expression profile during illumination to identify light-regulated sORFs and will report the microarray results in this meeting.
