Host: The Japanese Society of Plant Physiologists
Pages 0076
It has been reported that the plasma membrane of animal cell has lipid microdomains in which particular lipids and proteins are assembled. Microdomain is likely to contribute for the signal transduction and membrane transport. Recently, also in plant cells, the presence of plasma membrane microdomain has been indicated.
In a plant cell, many kinds of membrane transporters are involved in various vacuolar functions, but the distributions of these transporters on the vacuolar membrane are remaining unknown.
In the present study, to analyze distribution of proton pumps on the vacuolar membrane, we isolated intact vacuole from Arabidopsis suspension cultured cells, and stained proton pumps with immunofluorescent method. We found that v-ATPase was assembled in small area on isolated vacuole, but v-PPase was dispersed. Detergent resistant membrane (DRM) prepared from the vacuolar membrane contained abundant v-ATPase. v-PPase was contained much in detergent soluble membrane (DSM) than in DRM.
