Abstract
Compensation is a phenomenon in which a decrease in cell number triggers an increase in mature cell size. This phenomenon is important to understand the coordination of cell proliferation and expansion during organogenesis. We have isolated and reported our analysis on five fugu mutants that exhibit compensation. Here, we report about the fugu2 mutant. Map-based cloning revealed that FUGU2 was FASCIATA1, which encodes for the chromatin assembly foctor-1 p150 subunit. To elucidate the molecular mechanisms of compensation, we performed micro-array analysis using mRNA extracted from leaf primordia of fugu2-1 and confirmed the results by RT-PCR. In fugu2 leaves, the expression of 46 genes was induced and that of 39 genes was repressed more than three fold. Particularly, 11 among the induced genes were involved either in DNA replication or repair. By using T-DNA insertional lines, we are now investigating relationships between compensation and these changes of gene expression.
