Abstract of the Annual Meeting of JSPP 2009
Conference information
Host: The Japanese Society of Plant Physiologists
How Does ROT4 Peptide Regulate Growth of Arabidopsis?
Pages 0108
Details
Host: The Japanese Society of Plant Physiologists
Pages 0108
The ROT4 (ROTUNDIFOLIA4) gene is widely conserved among land plants. In addition, constitutive overexpression of ROT4 in Arabidopsis exhibits pleiotropic phenotypes, such as shorter lateral organs, shorter statures, shorter roots, and protrusion at the base of pedicels and trichomes. Thus, it is suggested that ROT4 plays important roles in plant development, however, the molecular function is largely unknown.
To get insights about the functions of ROT4, we first generated a series of truncated version of the coding region and examined the phenotype of these overexpressors. As a result, we found that highly conserved region of 32 amino acid residues was necessary and sufficient for the ROT4 functions. We generated chimeric overexpressor of GFP:ROT4 using HSP::Cre/Lox system, and found that GFP:ROT4 functions in an autonomous fashion. We also found interesting phenotypes of chimeric transgenic plants. In this presentation, we will discuss the function of ROT4 on plant development.
