Abstract
In the fertilization process of angiosperms, pollen tubes are not gathered around an ovule, but a single pollen tube is usually guided to each ovule. Such pollen tube guidance is considered to be involved in the mechanism that ensures blocking polyspermy.
We have recently identified small proteins as candidates of pollen tube attractants derived from the synergid cell of Torenia fournieri. The aim of this study is to investigate relation between the blocking polyspermy and attractants, by visualizing the candidates with immunological methods. We could establish methods for western blot analysis and immunostaining to show the candidate small proteins were secreted from mature ovules. We will show detailed time course analysis to discuss about the relation with blocking polyspermy.
