Host: The Japanese Society of Plant Physiologists
Pages 0141
Plant body size is thought to be tightly regulated by nuclear ploidy levels and cell numbers.
sd5 mutant was isolated as a dwarf and seedling lethal mutant from RIKEN Ds insertional mutant lines. Cell numbers rather than ploidy levels were decreased in sd5 cotyledons and CYCB1;1 expression was also down-regulated, indicating that cell proliferation was strongly affected in sd5.
Corresponding gene of sd5 encodes a homologue of yeast DIM1, a component of U5 spliceosome. Loss of DIM1 caused lethality as a result of defect of cell cycle in S. pombe. Interestingly, SD5 did not complement to the pombe dim1-35 mutation when SD5 was expressed under limited temperature. On the other hand Arabidopsis another homologue of DIM1 could complement to this mutation. These results suggested SD5 plays different functions of authentic DIM1 protein.
Here we will demonstrate the detail of sd5 phenotypes and physiological functions of SD5.