Abstract
Among 2,090 chloroplast proteins identified by computational prediction in Arabidopsis, we collected 3,244 tag-lines disrupted by transposon or T-DNA that encode 1,369 chloroplast proteins. From this collection, we have not only collected 1,234 homo-lines without visible phenotypes, but also isolated 100 mutants showing abnormal seedling (apg mutants) and 141 mutants of which homozygous lines were not obtained. We are constructing the RIKEN Arabidopsis Chloroplast Encyclopedia (RACE) database that presents genetic and phenotypic information on our resources, as well as essential, non-redundant genes that show an abnormal phenotype at seedlings and developing seeds stages when disrupted by mutation. The RACE database also provides tools for searching by AGI code, mutant line number and phenotype, and rapid access to detailed information on the resources. Moreover, our homozygous collections can be expected to yield powerful tools to accelerate and enhance the functional genomics of nuclear-encoded chloroplast proteins in Arabidopsis.
