Isolation and Characterization of Genes Encoding ACT Domain Containing Proteins (ACT1 and ACT2) in Arabidopsis

Abstract

We have isolated and characterized of novel genes encoding ACT domain containing proteins, ACT1 and ACT2, from Arabidopsis. ACT1 and ACT2 contain an ACT domain, an amino acid-binding domain. The domain is a regulatory domain mainly found in proteins involved in amino acid and purine metabolism. RT-PCR analysis indicated that the expression pattern of ACT1 and ACT2 genes was deferent in various organs from Arabidopsis. ACT1 mRNA was accumulated mainly in rosette leaves. In contrast, ACT2 mRNA was accumulated mainly in roots. To understand a role of ACT1 and ACT2, we are examining the characterization of Arabidopsis mutants ( act1 and act2 ) defective in ACT1 and ACT2 gene products.