Establishments of novel lines for functional analysis of transcription factors and analysis of hypo-sensitive mutant to light irradiations isolated from these lines

Abstract

We are establishing large population of novel lines independently overexpresing each transcription factor for functional genomics in Arabidopsis. We have employed glucocorticoid receptor (GR)-mediated functional induction system to avoid lethality caused by overexpression of some transcription factors. We have isolated a mutant that shows long hypocotyls phenotype by DEX application under long day condition. The long hypocotyls phenotype was observed under blue, red and far-red light conditions, but not under dark conditions. The gene encodes zinc finger type transcription factor, and transcriptional repression domain was predicted near C-terminal. These results suggest that the novel transcription factor function as negative regulator in light signal transduction of this mutant. Presently, we are producing knockdown mutants of this gene, because T-DNA insertion mutant of this gene does not exist.