Abstract
Lipid metabolism in flower is distinct from that in leaves and potentially involved in many aspects of flower development or reproductive processes. In Arabidopsis, however, lipid metabolism in flowers is largely unknown mainly because flowers are too tiny to be sampled according to the developmental stages. Here, we established Arabidopsis Super Plant, which shows synchronized flower development, as a tool for flower lipidomics analysis. By introducing inducible APETALA1 transgene into ap1-1cal-5, whose flower development is arrested, flower development was synchronized till the anthesis. Using this system, we conducted lipidomics analysis on membrane lipids in different developmental stages. The results revealed that lipid contents were dynamically changed during flower development. These results suggest that lipid metabolism is actively regulated by induction of distinct set of gene during flower development, and that Super Plant is a powerful tool to investigate flower lipid metabolism in Arabidopsis.
