Abstract
Proline accumulates under osmotic stress and acts as an osmolyte in plant. Proline biosynthesis occurs via two pathways from either glutamate or ornithine, proline biosynthesis from glutamate, in which Δ1-pyrroline-5-carboxylate synthetase (P5CS) is the key enzyme, appears to be the predominant pathway under stress conditions. Investigating the gene expression of P5CS in rice, we attempted to overexpress the gene expression of P5CS in rice. In the transgenic rice overexpressing P5CS mRNA, Pro content was increased six fold higher than the wildtype. We treated the rice cells with salts (NaCl or CaCl2). After 17 hours of 200mM NaCl treatment, the viability of the transgenic cell was higher than the wildtype moreover, after 6 hours of 400mM NaCl treatment significant difference was detected. Similar results were detected on CaCl2 treatment. In addition, the growth of their callus on the solid medium containing 100mM NaCl was the significant difference.
