Abstract
Plant causes immune response after perception of flagellin which is component of bacterial flagella filament. Previously FLS2 was identified as flg22 receptor in Arabidopsis thariana. Flagellin recognition system in rice, however, is still not known. Therefore we examined the flagellin perception system in rice. Rice recognizes two domains, flg22 and C-terminal region in flagellin. We attempted to identify receptor for flg22 in rice and identified OsFLS2, ortholog of FLS2. When OsFLS2 was overexpressed in rice cells, ability of flg22 perception was increased. OsFLS2 could complement A. tharianaΔFLS2 mutant, suggesting that OsFLS2 is functional for flg22 perception. To identify the receptor for C-terminal of flagellin, microarray analysis was performed. Among the identified gene, we concluded that STR1 and STR2 are candidate for the receptor. When both genes were overexpressed in rice, flagellin perception ability was increased. Moreover, STR1 and STR2 deficient mutants lost the ability of C-terminal flagellin perception.
