Abstract
Tomato is an excellent model plant for studying development, ripening and metabolism of fruits. The International Solanaceae Project (SOL, www.sgn.cornell.edu) was initiated by members from more than 30 countries in 2003, and the tomato genome projects are currently underway.
As a contribution of the Japanese SOL (JSOL) to the international collaboration, we started generating T-DNA tagged lines using a miniature tomato cultivar Micro-Tom. T-DNA insertional mutagenesis is a complementary approach to that by EMS and provides a rapid way to isolate mutated genes. More than 80 independent lines transformed with T-DNAs were generated. Southern blot analysis indicated that average copy number of T-DNA per the transgenic plant was 1.86. Of 109 T-DNA flanking sequences obtained, 60 sequences correspond to those from the Micro-Tom genome. We will show a current status of this project including some visible phenotypes of the T-DNA tagged lines and map-positions of the T-DNA insertions.
