Abstract
Photoperiods control several morphogenic responses such like flowering throughout plant life cycle. Recently, Hd3a was shown to be an important component of the mobile florigen signal. To study the function and localization of Hd3a in rice, we expressed the Hd3a-GFP gene by fusing it with phloem specific Hd3a promoter. Transgenic rice plants flowered significantly earlier than wild type, suggesting that expression of Hd3a-GFP causes early flowering. To examine tissue localization of the Hd3a in Hd3a-GFP transgenic plants, we analyzed GFP fluorescence in the SAM by confocal laser scanning microscopy. The GFP signal was detected in the SAM. There are some mechanisms that regulate the movement of Hd3a from the vascular bundles into the SAM. But there mechanisms are unclear. So, we study how Hd3a is translocated from the vascular bundles into the SAM and Hd3a regulated in the SAM.
