Abstract
In the previous study, we discovered a novel NDH-PSI supercomplex in wild-type Arabidopsis plants and its intermediate supercomplex in the NDH mutants lacking NdhL and NdhM. To further investigate the components of two supercomplexes, we excised the bands containing them from BN-PAGE and used them for LTQ-Orbitrap Mass analysis. The result showed that supercomplex included PSI subunits and NDH complex, while only the soluble NDH subcomplex was missing in the intermediate. To study further the physiological function of the supercomples, we are investigating whether the supercomplex formation is essential for the stability and/or activity of the NDH complex.
In addition, our proteomics analysis also identified several NDH subunit candidates including PPL2, NDF1, NDF2, NDF4 and NDF6 which have been reported recently. So far, two novel NDH subunits, including one FKBP domain containing protein and one transmembrane protein, were further confirmed to be novel NDH subunits by genetics approach.
