Abstract
We have investigated the effects of gibberellin (GA) in shoot on the gene expression in root. We have performed microarray analysis of GA-deficient mutant of Arabidopsis thaliana (ga3ox1/ga3ox2), which was grown for 3 weeks, applied by distilled water or GA4 on the shoot, and the root sample was collected 3 days after the hormone treatment. The gene expression level in root was analyzed with or without GA by Real-time PCR and RT-PCR analysis. In this research, we have focused on some metal absorption-related genes which were up-regulated by GA treatment, and compared the gene expression level in root between WT (Col) and GA-deficient mutant. In addition, the gene expression level of some transcription factors was found to be also up-regulated by GA treatment. Those results suggest that GA from shoot affects the gene expression of some metal transporter in root.
