Abstract
Next generation sequencing technologies, or deep sequencing, is revolutionizing the genomics. Our group is exploiting the 454 Sequencer by Roche, Switzerland, installed in Functional Genomics Center Zurich (FGCZ). A run of The 454 Sequencer yields >1,000,000 fragments with read length of 400 bp, which is the longest in next-generation sequencers. The 454 Sequencer has been exploited for the identification of microbial community and metagenomics, because it can avoid the significant bias of culture or cloning process during Sanger sequencing. We have surveyed bacterial community living within carnivorous plants. We also exploit the 454 Sequencer for the molecular analysis of so-called "non-model" plants, in which prior genomic information is limited.
