Abstract
The chloroplast NAD(P)H dehydrogenase(NDH) complex reduces plastoquinones and generate H+ gradient using NAD(P)H in photosystem I cyclic electron flow and chlororespiration. The NDH complex is found in land plants and cyanobacteria, and a number of NDH-related genes were characterized, while whole machinery of NDH is not identified yet. Last few years, we have succeeded to identify novel six genes related to NDH activity, NDF1, NDF2, NDF4, NDF5, NDF6 and PPL2, using a co-expression analysis of Arabidopsis. Among NDF genes, NDF2 and NDF5 showed weak homology to each other.
Here we report that Physcomitrella has a single homolog of both NDF2 and NDF5, while both of them are conserved in higher plants. Functional roles of these proteins in NDH activity are investigated in immunoblot analyses in a panel of ndh mutants, using antibodies against NDH related proteins containing NDF2 and NDF5.
