Abstract
Deep supercooling (DSC) ability of the parenchyma cells of xylem (XPCs) is essential for freezing resistance of boreal woody plants in winter. DSC ability is induced during seasonal cold acclimation in XRCs with various physiological changes. In XRCs of larch (Larix kaempferi), changes in gene expression and sugar accumulation have been characterized in association with changes in DSC ability. However, roles of proteins on DSC ability are still unclear. Therefore, we examined to identify proteins that are related to the DSC ability. In parallel with the decrease in DSC ability of larch twigs of winter by artificial deacclimation, decrease in levels of sixteen winter-induced proteins were detected by two-dimensional electrophoresis. These proteins were excised from gel spots, digested with trypsin, and analyzed LC-MALDI MS/MS. Database search based on de novo sequences from MS/MS spectra revealed that some candidate proteins for increasing DSC ability were similar to several proteins.
