Abstract
Thellungiella halophila is a model halophyte with a small plant size, short life cycle, and small genome. Although it is a close relative of Arabidopsis, it shows not only extreme salt tolerance, but also chilling-, freezing-, and oxidative-stress tolerances, suggesting that Thellungiella is a good genomic resource for understanding abiotic stress tolerance. We had generated a full-length cDNA enriched library from various tissues and abiotic stress treated Thellungiella. To elucidate the functions of a large population of Thellungiella genes and to search efficiently for salt tolerance genes, we have been taking advantage of the Full-length cDNA Over-eXpresser gene (FOX) hunting system. We are working on two kinds of FOX hunting, a whole genome FOX hunting and mini-scale FOX huntings using Arabidopsis. In the mini-scale FOX hunting, we extracted 433 genes including abiotic stress inducible genes from the library. We will introduce our recent progress of the mini-scale FOX hunting.
