Abstract
It has been suggested that FtsH proteases are abundant in the stroma thylakoids and the grana margin because these domains are the site of repair of the damaged D1 protein in the thylakoids. In the present study we assayed the relative amount of the protease in the thylakoids, grana, stroma thylakoids, PSII membranes and PSII core. The active FtsH protease forms hexameric ring structure, but it is not clear whether the hexameric proteases are present in every membrane domain (fraction). We analyzed the structure of the FtsH proteases under control dark conditions, as well as heat or light stress conditions, using clear native gel electrophoresis. This work was supported by the JSPS fellowship (DC2).
