Abstract
Effects of light on expression of one of the glycolytic enzymes, fructose-1.6-bisphosphate aldolase (fbaA) were investigated in Synechocystis sp. PCC6803. The fbaA mRNA was induced by light pulse of 5 min in the presence of glucose, while glucose addition in the dark was not effective for induction of fbaA. Therefore, both light and glucose were required for the full induction of fbaA transcription. One of the response regulators, sll1330, was demonstrated to be involved in regulation of fbaA gene expression by light pulse and glucose. The transcript of fbaA appeared only slightly after light irradiation in the presence of glucose in the disruptant of this gene, while continuous light effectively induced transcription of fbaA. These results suggest that fbaA gene expression is controlled through two signal pathways: presumably photosynthetic processes and light pulses in the presence of glucose.
